Figure 1
From: KATP channel subunits in rat dorsal root ganglia: alterations by painful axotomy
Expression of K ATP channel subunits in DRG at the mRNA and protein level. A. RT-PCR product bands consistent with the presence of mRNA encoding K ATP channel subunits in DRG from control rats. Total mRNA was isolated from the L5 DRG. Amplified products appeared at positions corresponding to the expected base pair lengths of 168 (Kir6.2), 182 (SUR1), 110 (Kir6.1), 124 (SUR2) and 315 (18S). B. Western blotting using antibodies against K ATP channel subunits in DRG of control rats. Kir6.2 antibody recognized one immunoreactive band at 37 kDa. SUR1 antibody revealed prominent immunoreactive bands at 150 and 37 kDa, and a less pronounced band at 125 kDa. The band at 150 kDa indicates glycosylated SUR1 bound to Kir6.2. Kir6.1 did not detect any band in DRG tissue, while a band of 37 kDa was detected in samples of brain tissue. Two immunoreactive bands at 150 and 74 kDa were detected by SUR2 antibody. Molecular size markers are shown on the left.