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Figure 6 | Molecular Pain

Figure 6

From: KATP channel subunits in rat dorsal root ganglia: alterations by painful axotomy

Figure 6

Distribution of anti-SUR1 immunofluorescence in the subpopulations of NF200+ and NF200- neurons in control (A-D) and SNL (E-H) DRG. A, E: Red anti-SUR1 immunofluorescence is observed on plasma and nuclear membranes, satellite glial cells, and along the peripheral nerve fibers. White arrows point to SLI. These are more intense and funnel shaped in controls (A) compared to SNL (E), wherein SLI loose their characteristic funnel shape, and appear less intense, thin and disorganized. B,F. Anti-NF 200 staining (green), distinguishes two DRG neuronal subpopulations, corresponding to larger, myelinated NF200+ and to smaller, non-myelinated NF200- fibers. C,G. Merged images showing the difference in distribution of SUR1 staining in each neuronal subgroup between SS and SNL DRG. SLI are shown with arrows in C. D, H: Bright field images. SLI are shown by yellow arrowheads in D. I(1-4). Bargraphs showing the differences in the prevalence of SUR1+ staining between control (C) and SNL NF200+ (I1) and between control (C) and SNL NF200- neuronal somata (I2), compared by Fisher's exact tests. I3. Bargraphs showing the decreased prevalence of SUR1+ SLI between control (C) and SNL axons, compared by Fisher's exact test. I4. Scatterplots showing the difference in the intensity of SUR1 immunofluorescence in SLI between axons from SNL versus control DRG. Arbitrary fluorescence units are used. Comparisons were made by Student's t test.

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