Dose response effect of ZJ43, peptidase inhibitor, on evoked current in control mouse CeLC region. (A) Representative evoked current traces shown in upper panel of ZJ43 at each concentration in a CeLC neuron; lower panel shows a representative cell current output traces over the same time duration as dose response experiments without a significant rundown in magnitude. (B) Average values for ZJ43 dose response effect on evoked excitatory currents in the CeLC of the untreated control mouse brain slice (n = 4). ZJ43 (10 μM) resulted in maximal (~50%) reduction in the amplitude of the evoked current. ZJ43's inhibitory effect at 100 μM (p < 0.01), 10 μM (p < 0.02) and 1 μM (p < 0.01) were significantly more potent compared to its effect at the minimum dose 10 nM. Data presented as mean ± SEM of percent control values, *p ≤ 0.05 vs. control, #p ≤ 0.05 vs. 10 nM ZJ43, one way RM-ANOVA followed by pairwise Tukey's t-test post hoc analysis. Recordings were in Mg2+ free ACSF.