Figure 4From: Endogenous N-acetylaspartylglutamate (NAAG) inhibits synaptic plasticity/transmission in the amygdala in a mouse inflammatory pain model Dose response effect of ZJ43, peptidase inhibitor, on evoked current in control mouse CeLC region. (A) Representative evoked current traces shown in upper panel of ZJ43 at each concentration in a CeLC neuron; lower panel shows a representative cell current output traces over the same time duration as dose response experiments without a significant rundown in magnitude. (B) Average values for ZJ43 dose response effect on evoked excitatory currents in the CeLC of the untreated control mouse brain slice (n = 4). ZJ43 (10 μM) resulted in maximal (~50%) reduction in the amplitude of the evoked current. ZJ43's inhibitory effect at 100 μM (p < 0.01), 10 μM (p < 0.02) and 1 μM (p < 0.01) were significantly more potent compared to its effect at the minimum dose 10 nM. Data presented as mean ± SEM of percent control values, *p ≤ 0.05 vs. control, #p ≤ 0.05 vs. 10 nM ZJ43, one way RM-ANOVA followed by pairwise Tukey's t-test post hoc analysis. Recordings were in Mg2+ free ACSF.Back to article page