Endogenous NAAG acts presynaptically to reduce the frequency of mEPSCs in the CeLC region. Application of ZJ43 (0.1 μM) in the presence of TTX and CPP (to block action potentials and NMDA current respectively) in the CeLC region of the amygdala in slices from untreated control mice significantly reduced the frequency of miniature EPSC, while the amplitude of the mEPSCs was not significantly altered. A) Representative current traces in the absence and presence of ZJ43 in a neuron. (B) Cumulative amplitude curve in the presence and absence of ZJ43. (C) Cumulative inter-event interval curve depicting mEPSCs frequency rate in the presence and absence of ZJ43 in a neuron from a control untreated mouse brain slice. Bar graph showing % control of amplitude (p = 0.3, n = 9, ZJ43 treated versus control) (D) and frequency (E) values for cells (n = 9). Data presented as mean ± SEM, *p ≤ 0.05 vs. control, paired Student's t-test. Recordings were in Mg2+ free ACSF.