Figure 7

ZJ43 inhibit the evoked current in CeLC neurons 6 hours after induction of peripheral inflammation. The group II mGluR antagonist, LY341495 (1 μM), was used to define the level of tonic activation of the receptors as a consequence of peripheral inflammation. Representative traces: (A) ZJ43 (0.1 μM) significantly reduced eEPSCs in CeLC neurons; Average data showing (D) ZJ43 effects 6 hours post inflammation (n = 7). (B) In a separate set of cells, the direct effect of LY341495 was tested on the evoked current in the brain slice 6 hours post formalin treatment; (C) Average data showing LY341495 effect 6 hours post inflammation (p = 0.17, n = 7). Data presented as mean ± SEM percent control; *p ≤ 0.05 vs. control, paired Student's t-test. Recordings were in Mg²⁺ free ACSF.