Expression of PAR1-4 in sections of adult mouse DRG. A. In situ hybridisation (ISH) for PAR1-4 carried out as described in Methods. Positive cells shown by arrowheads. Sections counterstained using hematoxylin-eosin. Scale bars 40 μm.
B. Similar sections in which PAR1, 3 and 4 expression was determined using immunohistochemistry. Positive cells shown with arrows. The PAR2 antibodies available to us were found to be non-specific on Western blots and results for PAR2 are therefore not shown. Sections counterstained using hematoxylin-eosin. Scale bars 40 μm.
C. Expression of PAR1 - 4 as a function of neuronal size in adult mouse DRG using ISH. Overall neuronal population (grey) is compared with those positive for each PAR isoform (black). Overall, PAR1 was found to be expressed in 15.0% of neurones, PAR2 in 21.5%, PAR3 in 49.5% and PAR4 in 14.5%.
D. Similar results obtained using immunohistochemistry. PAR2 is not shown because the antibody was found to exhibit non-specific binding, and PAR4 is not shown because it proved impossible to distinguish neuronal from glial cell staining (see B). Overall PAR1 was expressed in 10.3% of neurones, and PAR3 in 42.0%.