Figure 5
Role of caspase-1 in the induction of COX-2 and prostaglandin production during carrageenin-induced inflammation. (A) Wild type and casp1-/- mice received an intraplantar injection of carrageenin (100 μ/paw) or saline. After 3 h, plantar tissue samples were removed and the expression of COX-2 was determined by western blot. The β-actin level was used as a control. Data are presented as representative blots. (B) Densitometry of the pixel intensity of COX-2 bands relative to β-actin is present. (C) Wild type and casp1-/- mice received an intraplantar injection of carrageenin (100 μ/paw) or saline. After 3 h, plantar tissue samples were removed and the level of PGE2 was determined by RIA. Data are expressed as the mean ± S.E.M. of 5 animals per group. * indicates statistical significance compared to the saline-injected group; # indicates statistical significance compared to the wild type group. P < 0.05, one-way ANOVA followed by the Bonferroni's test.