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Figure 1 | Molecular Pain

Figure 1

From: Expression of inwardly rectifying potassium channels by an inducible adenoviral vector reduced the neuronal hyperexcitability and hyperalgesia produced by chronic compression of the spinal ganglion

Figure 1

The structure and method of application of the viral vectors. A: Schematic representation of the ecdysone-inducible adenovirus vectors and method of application to the DRG. ITR: inverted terminal repeat; ψ: Packaging signal; Ecd promoter: ecdysone-inducible promoter; EGFP: enhanced green fluorescent protein; IRES: internal ribosome entry site; MCS, multiple cloning site; pA, SV40 polyadenylation signal; DBEcR, hybrid ecdysone receptor. AdCDBEcR: receptor virus. AdEGI: the control vector containing only the EGFP gene. AdEGI-Kir2.1: the viral vector containing both the EGFP and the Kir2.1 gene, which encodes an inward-rectifying potassium channel (See main text and Johns et al. 1999 [9] for further details). B: Procedures for sub-epineurial injection of viral vectors into the L4 DRG and rod implantation. Under a dissecting microscope, the L5 transverse process was removed to expose the L4 spinal nerve. A polyethylene tube (tip diameter 100 μm) connected to a microinjection syringe was inserted into the L4 spinal nerve under the epineurium until the tip reached the center of DRG. The viral vectors or vehicle were slowly injected into the sub-epineurial space of DRG using a microinjection pump (5 μL in about 10 min). For CCD surgery, a stainless steel rod was inserted into the intervertebral foramen to compress the L4 DRG.

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Molecular Pain

ISSN: 1744-8069

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