Figure 2
Behavioral effects of the adenoviral vector in the absence of CCD. Either the vehicle, the control vector (AdEGI), or the vector containing the Kir2.1 gene (AdKir) was delivered to the L4 DRG via a sub-epineurial injection. The induction agent was then delivered, IP (arrow), either immediately (postoperative day 0) (A) or after a delay of three days (B). In either case, a second induction was delivered on the 10th postoperative day. FWT: Mean threshold force (mN) for foot withdrawal. Injection of vehicle alone produced on the ipsilateral foot, a transient mechanical hyperalgesia (significant decrease in FWT from preoperative values) that recovered within 5 days (A). Ipsilateral (Ipsi) but not contralateral (Con) to the CCD, an injection of vehicle containing either AdEGI or Adkir, produced a mild and long-lasting mechanical hyperalgesia regardless of whether the inducing agent was given immediately or after a delay of 3 days suggesting that the hyperalgesia produced by the presence of virus was not reduced by the expression of Kir. *: P < 0.05 vs. pre-operative mean for the ipsilateral foot of AdEGI group and AdKir groups (A and B); †: P < 0.05 vs. pre-operative mean for the ipsilateral foot of Vehicle group (A).