Figure 1
From: Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice
Quantitative real-time PCR analysis of tTA and tetO lines. Real-time quantitative PCR was used to measure: A, the fold increase in the level of tTA mRNA in pooled DRG samples one week after axotomy. Line 176 has significantly higher levels of tTA mRNA than tTA9 and tTA17 which do not differ significantly from each other or WT mice. B, expression of total galanin mRNA in pooled DRG samples from intact transgenic and WT mice were compared. Line tetO21, tetO36 and tetO82 were increased by 5.36, 1.69 and 1.44-fold respectively. Line tetO21 was significantly different to the other 2 lines (1-way ANOVA, *** denotes P < 0.001).