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Figure 3 | Molecular Pain

Figure 3

From: Z-360, a novel therapeutic agent for pancreatic cancer, prevents up-regulation of ephrin B1 gene expression and phosphorylation of NR2B via suppression of interleukin-1 β production in a cancer-induced pain model in mice

Figure 3

Effect of Z-360 on spinal cord NR2B phosphorylation in the mouse model of cancer-induced pain. A. NR2B was immunoprecipitated from lysates of the spinal cord of cancer-induced pain model in mice. The immunoprecipitates were separated on 10% SDS-PAGE gels and then transferred onto PVDF membranes with a Trans-Blot Cell System. The membranes were first probed with an antibody against pTyr1472-NR2B, stripped, and then reprobed with an antibody against NR2B. The panels show representative immunoblots of normal control, vehicle control, and Z-360 (100 mg/kg) treatment performed with antibodies against pTyr1472-NR2B (top) and NR2B (bottom). B. Histograms showing the relative tyrosine phosphorylation of NR2B normalized to normal controls. Adobe Photoshop Elements 5.0 software was used for densitometric quantification. Values represent the mean ± S.E. (n = 3). #, p < 0.05, normal versus vehicle (t-test); *, p < 0.05, vehicle versus Z-360 (t-test).

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