Figure 1

Exon 2 of ephrin-B2 was deleted in DRG in Efnb2 CKO mice. (A) Diagram showing the ephrin-B2 wild-type locus, the targeted locus before and after Cre excision, Nav1.8 wild-type and Nav1.8-Cre locus. Exons are represented as numbered boxes. The loxP site (blank triangles) and the PCR primers (black arrows) are indicated. (B) Genotyping analysis with PCR. The DRGs genomic DNA from Efnb2 CKO mice, Efnb2^fl/fl controls, heterozygous floxed exon 2 ephrin-B2 (Efnb2^fl/+) mice and C57BL/6 wild-type (WT) mice were examined with PCR. The wild-type band (wt), floxed band (floxed) and Cre band (cre) were amplified with primer sets a/b, a/c and d/e respectively. The knockout band (ko) was found only from Efnb2 CKO mutant mice.