Figure 3

Acute pain behavior was not affected in ephrin-B2 mutant mice. (A) Rotarod studies showed no motor deficits in Efnb2 CKO animals. (B) Responses to low-threshold mechanical stimulation by von Frey filaments are normal in Efnb2 CKO mutant mice. (C) Acute mechanical pressure applied with the Randall Selitto apparatus demonstrated identical behavior in Efnb2 CKO and Efnb2^fl/fl mice. (D) Hargreaves' apparatus also demonstrates identical latencies of response to thermal stimulation in Efnb2 CKO and Efnb2^fl/fl mice. (E) Supra-spinal reflexes to heat (50 and 55°C) using the hot plate apparatus demonstrates identical latencies in Efnb2 CKO and Efnb2^fl/fl mice. (F) Cold behavior (number of liftings) is the same in Efnb2^fl/fl and Efnb2 CKO mice (0°C) using the cold plate apparatus.