Figure 4

Both inflammatory and neuropathic pain behavior are attenuated in ephrin-B2 null mutant mice. (A) Two phases (0 - 10 min, 10 - 60 min) of licking and biting behavior after intraplantar injection of formalin were analyzed, the second phase of which is attenuated in Efnb2 CKO mutant mice. (B) Intraplantar injection of carrageenan caused identical thermal hyperalgesia in Efnb2^fl/fl controls and Efnb2 CKO mice. (C) Intraplantar injection of carrageenan caused a long-term mechanical allodynia in both Efnb2 CKO mutants and controls. (D) Intraplantar injection of CFA caused identical thermal hyperalgesia in Efnb2^fl/fl controls and Efnb2 CKO mutant mice. (E) However, intraplantar injection of CFA caused a long-term mechanical allodynia that is attenuated after 4 hours in Efnb2 CKO mutants (two-way ANOVA, * p < 0.05). (F) Both Efnb2 CKO mutant mice and Efnb2^fl/fl controls showed thermal hyperalgesia after sciatic nerve ligation (Seltzer model). However, thermal hyperalgesia was attenuated in Efnb2 CKO mutants (two-way ANOVA, * p < 0.05). (G) Sciatic nerve ligation following the Seltzer procedure caused a long-lasting mechanical allodynia that was attenuated after 5 days of surgery in Efnb2 CKO mutant mice (two-way ANOVA, * p < 0.05).