receptor-dependent and -independent down-regulation of MAG. (a and b) The MAG expression following nerve injury in the dosal root (DR), spinal nerve (SPN) and sciatic nerve (SCN) fibers in WT or Lpar1-/- mice at day 7 post-injury, were assessed by immunohistochemical analysis (a) and western blot analysis (b). (a) Photographs show representative data. Scale bar, 20 μm. (b) Immunoreactive signals for MAG (100 kDa) and β-tubulin (β-Tub; 55 kDa) were detected. (c) Time-course of MAG down-regulation in the dorsal root after injury. (d) Comparison of MAG expression levels between uninjured WT and Lpar1-/- mice. Photographs show a representative image of a western blot. Data were obtained from three independent experiments.