LPC-induced dorsal root demyelination mediated through ATX. Toluidine blue analysis was performed at 24 h after the addition of 100 ng/ml of LPC to ex vivo cultures of DR fibers in the presence or absence of ATX (30 ng/ml). (a) Photographs show representative data. Scale bar, 10 μm. (b) Quantification of demyelinated fibers. Results are calculated as a percentage of total fibers, and expressed as the mean ± SEM from 3-5 independent experiments. *p < 0.05, vs. vehicle (Veh)-treated group.