LPC-induced dorsal root demyelination mediated through ATX and LPA
receptors in vivo. Toluidine blue analysis was performed at day 4 after the intrathecal injection of LPC (15 μg) in WT, atx+/- or Lpar1-/- mice. (a) Photographs show representative data in the dorsal root. Scale bar, 10 μm. (b) Quantification of demyelinated fibers. Results are calculated as a percentage of total fibers, and expressed as the mean ± SEM from 3-5 independent experiments. *p < 0.05, vs. WT and vehicle (Veh)-treated mice and #p < 0.05, vs. WT and LPC-treated mice.