Figure 4

Temporal profile of microglial and astrocyte activation following ligation of the mental nerve. Microglia and astrocytes were immunolabeled with Iba-1 and GFAP, respectively. The intensity of Iba-1 immunoreactivity (ir) in Sp5C area was increased between days 3-days 14 and sharply diminished after days 28 post-injury whereas GFAP immunoreactivity increased significantly after days 14 and remained elevated at least until days 28. Scale bar: 50 μm.