Figure 8

In situ hybridization detected robust expression of IκB-α and IL-1β mRNAs in the brain following local LPS injection. A). IκB-α mRNA was dramatically increased in the CVOs, such as SFO, meninges and ventricle related structures at 5 hours post LPS injection, and this increase was significantly reduced at 48 hours. No positive signals were detected within the parenchyma, including Sp5C. B). IL-1β mRNA was also induced in the CVOs, such as SFO, AP and choroid plexus at 5 hours post LPS injection, it was almost completely disappeared at 48 hours. No positive signals were detected within the parenchyma, including Sp5C. C). Double immunofluorescent labelling demonstrated that some of IL-1β⁺ cells are OX-42⁺ microglia. Scale bar: 500 μm in A and B; 50 μm in C.