Figure 4

GFAP immunoreactivity in the TREZ at day 8 after pIONL. (A), GFAP-IR in sham operated WT mice. (B, C), pIONL induced slight increase in the intensity of GFAP staining in the ipsilateral TREZ of WT mice but not the contralateral side. (D, E), KOR-/- mice or mice pre-treated with norBNI did not show significant upregulation of GFAP immunoreactivity in the ipsilateral TREZ, and this was the same intensity as in the contralateral TREZ of WT pIONL mice (B) or WT sham operated mice(A). (F), Mean ± SEM pixel intensity of GFAP immunoreactivity in TREZ at day 8 after pIONL. (*p < 0.001) Scale bars: A-E, 50 μm