Figure 6
From: Trigeminal injury causes kappa opioid-dependent allodynic, glial and immune cell responses in mice
Cell proliferation central trigeminal root at day 8 after pIONL. (A) Data came from Vp (the area shown in red box). (B, C, F), BrdU positive cells are markedly increased in the ipsilateral Vp inWT pIONL compare to contralateral side. (B-H), in KOR-/- and NBNI pre-treated pIONL mice, the number of BrdU positive cells significantly decreased compared to WT pIONL mice. (I-N), Different cellular markers (GFAP for satellite cells, CD11b for microglia, nestin for neuronal stem cell) were used to identify the phenotypes of dividing cells. The majority of BrdU positive cells were double labeled with nestin and CD11b. Only few BrdU positive nuclei were double labeled with GFAP. (*p < 0.001) Scale bars: A, 250 μm, C-H, 50 μm, I-N, 30 μm.