Gap 43 immunostaining in adult rat DRG neurons in vitro . A. without oxaliplatin treatment, DRG neurons are densely distributed and the cell bodies and neurites are intensely positive for Gap43 immunostaining (green), with long, robust and profusely branched neurites. B. Neurons treated with 5 μg/ml oxaliplatin have shorter, less dense neurites (double arrowheads), and reduced intensity of Gap43 immunostaining (double arrowheads). C. after 20 μg/ml and D. 50 μg/ml oxaliplatin treatment, there is a dose related loss of neurites (double arrowheads), cell bodies (single arrowheads) and loss of Gap43 immunostaining. Bar = 30 μm.