Figure 5

Reduced Extracellular Ca²⁺ Potentiates AGM-Induced ASIC3 Channel Activation. (A) Representative traces showing currents induced by reduced extracellular Ca²⁺ (left) and 1 mM AGM applied under low Ca²⁺ (right). (B) Pooled data as shown in (A). Data points are means ± S.E.M. of four to five measurements normalized to currents evoked with 0.01 mM Ca²⁺ (dashed line). Expected value is the linear summation of normalized currents induced by reduced Ca²⁺ and 1 mM AGM under low Ca²⁺ conditions individually. *p < 0.05, **p < 0.001. The two-way ANOVA analysis suggests the synergetic interaction between AGM and Ca²⁺ reduction (p < 0.01). (C) Representative traces showing currents induced by lactate (15 mM, keeping the pH neutral, light grey), AGM (1 mM) in the absence (black) or presence (dark grey) of 15 mM lactate. (D) Pooled data as shown in (C). Data points are means ± S.E.M. of five measurements normalized to AGM (1 mM)-induced currents (dashed line). Expected value is the linear summation of normalized currents induced by lactate (15 mM) and AGM (1 mM) individually. **p < 0.001.