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Figure 2 | Molecular Pain

Figure 2

From: Temporomandibular joint inflammation activates glial and immune cells in both the trigeminal ganglia and in the spinal trigeminal nucleus

Figure 2

GFAP immunoreactivity is increased in SGCs following induction of TMJ inflammation. (A, B) Few GFAP+ SGCs (red) encircling NeuN+ neurons (green) were detected in the contralateral (CONTRA) TG of CFA-injected rats, or in TGs from saline-injected rats (see below). A significant increase in the number of GFAP-encircled neurons was instead observed in the ipsilateral (IPSI) TG starting at 24 h post CFA injection (A', B'). Nuclei were labeled with the Hoechst 33258 dye (blue). Scale bars: 20 μm. (C-E) The number of GFAP-encircled neurons was increased in all 3 divisions (V1-ophthalmic, V2-maxillary, V3-mandibular) of ipsilateral (ipsi) TG. Similar, although smaller, changes were observed at 72 h p.i. Since no significant differences between saline-injected rats at 24 h and 72 h have been observed, data have been pooled together, and shown here as "saline". Data are expressed as number of GFAP-encircled neurons per counting field at 40x magnification, and refer to 2 independent experiments. *** p < 0.001 and * p < 0.05 compared to the contralateral side; one-way ANOVA.

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