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Table 1 Composition of extracellular and intracellular solution.

From: Alteration of primary afferent activity following inferior alveolar nerve transection in rats

V-clamp

 

I-clamp

 

Extracellular solution(mM)

   

   NaCl

30

   NaCl

155

   Choline chloride

50

   HEPES

10

   TEA

40

   KCl

3

   HEPES

10

   CaCl2

1

   MgCl2

3

   MgCl2

1

   Glucose

10

   Glucose

20

Adjusted to pH = 7.4 with TEAOH

 

Adjusted to pH = 7.3 with NaOH

 

Intracellular solution(mM)

   

   CsF

110

Methane Sulfonic Acid

135

   CsCl

40

   KOH

130

   TEA

40

   KCl

20

   HEPES

10

   NaOH

15

   NaOH

10

   EGTA

2

   EGTA

2

   HEPES

7.5

   MgCl2

2

  

Adjusted to pH = 7.2 with CsOH

 

Adjusted to pH = 7.2 with KOH or HCl

 
  1. TEA: Tetraethylammonium chloride, EGTA: ethyleneglycol-bis (b-aminoethyl ether)-N, N, N', N',-tetra acetic acid.
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Molecular Pain

ISSN: 1744-8069

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