Basal expression of GLAST is increased in glial cells in the TNC in response to GSE. Sections of spinal cord were obtained from untreated animals (CON) and GSE treated animals (GSE). (A) Images of spinal cord sections stained for GLAST are shown in the top panels. The bottom panels represent the same sections co-stained with GLAST and DAPI. (B) The average fold change ± SEM of GLAST staining intensity from control values is reported (n = 3 independent experiments) * P < 0.01 when compared to control levels. Magnification bar = 50 μm.