Figure 5

Blockade of mGluR1 reverses the pain-related increase of excitatory transmission and the decrease of inhibitory transmission in CeLC neurons. (A) A selective mGluR1 antagonist (LY367385, 10 μM) had no significant effect on I/O functions of EPSCs at the BLA-CeLC synapse in slices from normal rats (n = 3 neurons, P > 0.05, two-way ANOVA; see Results). (B) LY367385 inhibited excitatory transmission in slices from arthritic rats (n = 5 neurons, P < 0.0001, two-way ANOVA; see Results). (C) The inhibitory effect of LY367385 (normalized to predrug; set to 1.0) in the arthritis pain model was significantly different from that under normal conditions. * P < 0.05, unpaired t-test. (D) LY367385 had no significant effect on I/O functions of IPSCs in slices from normal rats (n = 3 neurons, P > 0.05, two-way ANOVA; see Results). (E) LY367385 increased inhibitory transmission in slices from arthritic rats significantly (n = 5 neurons, P < 0.0001, two-way ANOVA; see Results). (F) The facilitatory effect of LY367385 (normalized to predrug; set to 1.0) in the arthritis pain model was significantly different from that under normal conditions. * P < 0.05, unpaired t-test. (A-F) Symbols and error bars represent means ± SE.