Figure 8

Effects of MTEP on spontaneous and miniature excitatory and inhibitory postsynaptic currents in CeLC neurons from arthritic rats. (A) Original current traces of sEPSCs in an individual CeLC neuron (held at -70 mV) from an arthritic rat before (Predrug) and during MTEP (1 μM). (B, C) MTEP (1 μM) decreased sEPSC amplitude (C), but not frequency (B), significantly (cumulative amplitude distribution, P < 0.05, Kolmogorov-Smirnov test; mean amplitude, n = 5 neurons, P < 0.01, paired t-test). (D) Current traces of mEPSCs recorded in the presence of TTX (1 μM) in one CeLC neuron from an arthritic rat before (Predrug) and during MTEP (1 μM). (E, F) MTEP (1 μM) decreased mEPSC amplitude (F), but not frequency (E), significantly (cumulative amplitude distribution, P < 0.05, Kolmogorov-Smirnov test; mean amplitude, n = 5 neurons, P < 0.01, paired t-test). (G) Current traces of sIPSCs recorded in one CeLC neuron (held at 0 mV) in a brain slice from an arthritic rat before (Predrug) and during MTEP (1 μM). (H, I) MTEP (1 μM) decreased amplitude (I), but not frequency (H), of sIPSCs significantly (cumulative amplitude distribution, P < 0.05, Kolmogorov-Smirnov test; mean amplitude, n = 4 neurons, P < 0.001, paired t-test). (J) Current traces of mIPSCs in the presence of TTX (1 μM) in one CeLC neuron from an arthritic rat before (Predrug) and during MTEP (1 μM). (K, L) MTEP (1 μM) decreased amplitude (L), but not frequency (K), of mIPSCs significantly (cumulative amplitude distribution, P < 0.05, Kolmogorov-Smirnov test; mean amplitude, n = 4 neurons, P < 0.001, paired t-test). (A, D, G, J) Scale bars, 10 pA, 2 s. (B, C, E, F, H, I, K, L) Bar histograms show means ± SE.