Figure 1

Expression of DREAM, daDREAM and DREAM targets in spinal cord and dorsal root ganglia. (A) Quantitative real-time PCR analysis of the expression of dominant active DREAM (daDREAM) in spinal cord (SC) and dorsal root ganglia (DRG) in transgenic L1 and L26 mice. For comparison, expression levels of endogenous DREAM (endoDREAM) are shown. (B) Comparison of the expression of dominant active DREAM mutant in spinal cord at P7 and adult in wild type (wt) and L1 mice. (C) β-galactosidase positive cells show the distribution of the transgene in lamina I-II and X in the spinal cord of L1 mice. (D) Quantitative real-time PCR analysis of the expression of prodynorphin (Dyn) and brain-derived neurotrophic factor (BDNF) in spinal cord from wild type (wt) and transgenic L1 and L26 mice. (E) Quantitative real-time PCR analysis of the expression of BDNF mRNA and its receptor, tyrosine receptor kinase B (TrkB) in DRG and spinal cord from wild type (wt) and L1 mice. The mRNA levels in A, B, D and E are expressed as ratio to HPRT mRNA levels. Data are mean ± SEM (n = 6-9 in A and B and 5-7 in D and E). *** P < 0.001.