Figure 4
[3 H]-Morphine binding in WT and STOP null mice. A : Saturation analysis of [3H]-morphine binding to the MORs in wild-type and STOP null mice. Data are presented as means ± S.E.M. n = 3. B : Transformation of the data by linear regression_Scatchard plots. Data are presented as means ± S.E.M. n = 3. C : Comparison of the B max values of MORs in wild-type and STOP null mice. D : Comparison of the K d (C) values of MORs in wild-type and STOP null mice. Data are presented as means ± S.E.M. n = 3, Statistical significance between STOP null versus WT mice is indicated as follow: * p < 0.05, ** p < 0.01, *** p < 0.001 by Student t-test. E : Western blot analysis showing MOR immunolabel present in 50 μg of brain proteins of wild-type and STOP null mice (n = 3). No cross reactivity was found for the secondary antibody used.