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Figure 2 | Molecular Pain

Figure 2

From: A preconditioning nerve lesion inhibits mechanical pain hypersensitivity following subsequent neuropathic injury

Figure 2

Effects of preconditioning nerve lesion on PSNL-induced neuronal damage and inflammation in L4/5 DRGs. (A-B) Percentage of ATF3+ DRG neurons in populations of large NF-200-expressing neurons, and small peripherin-expressing neurons. Compared to the contralateral uninjured side, a large increase in NF-200 neurons (green) containing ATF3+ nuclei (red) (A) and in peripherin positive neurons (green) containing ATF3+ nuclei (red) (B) was observed on the side ipsilateral to PSNL in all groups. On the ipsilateral side, there was no significant difference between preconditioned (crush-injured) and unconditioned (no crush) groups. On the contralateral side, the percentage of NF-200 neurons containing ATF3+ nuclei (A) and peripherin neurons containing ATF3+ nuclei (B) was significantly higher in the rats that underwent right tibial nerve crush injury as compared to all other groups. (C-D) Macrophage and T-cell presence in DRGs. Compared to the contralateral uninjured side, ED1 immunoreactivity (in green) (C) and the number of T cells (in green) (D) were markedly increased after PSNL on the ipsilateral side, but with no significant difference between preconditioned and unconditioned groups. On the contralateral side, ED1 immunoreactivity (C) and T-cell numbers (D) were significantly higher in the rats that underwent right tibial nerve crush injury as compared to all other groups. Micrographs on the right of each histogram show representative examples of immunoreactivity in DRGs from injured (ipsilateral) and uninjured (contralateral) sides. (n = 3 rats per group, *P < 0.05, ** P < 0.01, *** P < 0.001, two-way ANOVA followed by Bonferroni post-tests). Data are expressed as mean ± s.e.m. Scale bars represent 50 μm.

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