The effect of thalassiolin B on the pH versus amplitude and steady state desensitization curve of the ASIC current. A) No significant influence of 100 μM thalassiolin B on the pH dependence of the ASIC current amplitude was found. B) The use of thalassiolin B also did not significantly modify the steady-state desensitization of ASIC currents. C) Analysis of the thalassiolin B effect as function of pH used to activate the current shows no significant correlation (P > 0.05). Line shows the linear regression (slope = 0.27); dotted lines show confidence intervals at P ≤ 0.05. D) Amiloride (30 μM) decreased the current 64 ± 4%. The coapplication of 30 μM amiloride and 100 μM thalassiolin B produced an additional nonsignificant 10.8 ± 3.8% decrease of the current (P ≥ 0.05 Student's t-test; thalassiolin B preapplied 20 s before coapplication of amiloride and thalassiolin B). Effects were fully reversible.