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Table 1 Solutions used for voltage clamp recording.

From: Antinociception produced by Thalassia testudinum extract BM-21 is mediated by the inhibition of acid sensing ionic channels by the phenolic compound thalassiolin B

 

Extracellular (mM)

pH 6.1 Extracellular (mM)

Intracellular (mM)

NaCl

140

140

10

KCl

5.4

5.4

140

CaCl 2

1.8

1.8

0.134

MgCl 2

1.2

1.2

-

HEPES

10

-

5

MES

-

10

-

EGTA

-

-

10

MgATP

-

-

2

Na-GTP

-

-

1

 

pH 7.4 adjusted with NaOH

pH 6.1 adjusted with NaOH

pH 7.2 adjusted with KOH

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Molecular Pain

ISSN: 1744-8069

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