Figure 1
From: Rapid S-nitrosylation of actin by NO-generating donors and in inflammatory pain model mice
Effect of NO donors on PACAP-stimulated dopamine release from PC 12 cells. A. Inhibition of PACAP-stimulated dopamine release by NO donors. PC12 cells (4 × 105 cells/well) cultured on 24-well dishes were preincubated for 30 min with NOR1, NOR3, SNAP or GSNO (100 μM concentration of each) and then stimulated with 10 nM PACAP for 5 min in the presence of 10 μM imipramine, an inhibitor of catecholamine reuptake. Dopamine released into the medium and cellular dopamine were measured by HPLC as described in "Methods." *P < 0.05; **P < 0.01 vs. without NO donor. B. Time course of inhibition of PACAP-stimulated dopamine release by NO donors. PC12 cells were stimulated with 10 nM PACAP in the absence and presence of 100 μM NOR1, NOR3 or GSNO, and 10 μM imipramine for the indicated times. *P < 0.05; **P < 0.01 vs. PACAP alone. C. Concentration dependency of NOR1 and NOR3 for the inhibition of PACAP-stimulated dopamine release from PC12 cells. PC12 cells were stimulated for 10 min with 10 nM PACAP in the presence of various concentrations of NOR1 or NOR3 and 10 μM imipramine. Released dopamine (mean ± SD, n = 3) was expressed as a percentage of total dopamine (7.2 ± 0.8 ng/well) in PC12 cells. *P < 0.05; **P < 0.01 vs. without NO donor. D. Inhibition by NO donors of the KCl-stimulated dopamine release in PC12 cells. PC12 cells (4 × 105 cells/well) cultured on 24-well dishes were preincubated for 30 min with NOR1, NOR3, SNAP or GSNO (100 μM concentration of each) in the presence of 10 μM imipramine and then stimulated with 45 mM KCl for 5 min. Dopamine released into the medium (mean ± SD, n = 3) was measured as described above and expressed as a percentage of total dopamine in PC12 cells. **P < 0.01 vs. without NO donor.