Biochemical characterization of PICK1 knockout (KO) mice. A, PCR analysis of tail DNA from wild-type (WT), heterozygous (HET), and PICK1 KO mice. M: molecular weight standard. B, Western blot analysis shows the expression of PICK1 protein and its interacting proteins ASIC1 and ASIC2 in the DRG from WT and PICK1 KO mice. C, Western blot analysis shows the expression of PICK1 and its interacting proteins GluR2 and PKCα in the spinal cord from WT and PICK1 KO mice.