PICK1 knockdown blunts neuropathic pain. L5 spinal nerve ligation (SNL) led to a significant increase in paw withdrawal frequency in response to 8.01 mN mechanical stimulation (A) and a marked decrease in paw withdrawal latency in response to heat stimulation (B) on the ipsilateral side in the saline-treated rats. Intrathecal administration of PICK1 antisense (AS) oligodeoxynucleotide (ODN, 10 μg) delayed the development of both mechanical and thermal pain hypersensitivities on days 3 and 5 post-SNL. No significant differences in paw withdrawal frequencies or latencies were observed between rats treated with saline and those treated with missense (MS) ODN (10 μg). ** P < 0.01 vs the same time points in the saline-treated group.