Figure 4
From: Spinal toll like receptor 3 is involved in chronic pancreatitis-induced mechanical allodynia of rat
Double immunostaining showing the cellular location of toll like receptor (TLR) 3 with antibodies against astrocytic specific marker glial fibrillary acidic protein (GFAP, A-D), microglial marker OX42 (E) and neuronal marker NeuN (F). We observed that TLR 3 was highly expressed on spinal astrocytes, and also expressed on microglia in a very low level. Single arrows: GFAP-positive and TLR 3-negative cell structures; Single arrowheads: GFAP-negative and TLR 3-positive cell structures; Double arrows: double-labeled cell structures. Scale bars = 200 μm in C, applied in A-C, 50 μm in F, applied in D-F.