Figure 5
From: Spinal toll like receptor 3 is involved in chronic pancreatitis-induced mechanical allodynia of rat
Intrathecal infusion of toll like receptor (TLR) 3 antisense oligodeoxynucleotide (ASO) on mechanical allodynia and astrocytic activation. TLR 3 ASO or mismatch oligodeoxynucleotide (MO) was intrathecally infused for 1 w after pancreatitis induction. (A) Response frequencies to mechanical stimulation of the abdomen with von-Frey filaments of various strengths at 5 w in the rats of trinitrobenzene sulfonic acid (TNBS)-MO, sham-MO, sham-ASO (0.1 nmol/μL) and TNBS-ASO (0.1 nmol/μL) group. *, # P < 0.05 compared with that of sham-MO or TNBS-MO group, respectively. (B) TNBS-induced allodynia was significantly attenuated by TLR 3 ASO in a dose-dependent manner. * P < 0.05 compared to 0.1 nmol/μL group. (C, D) Representative Western blot data showing the expressions of TLR 3 and glial fibrillary acidic protein (GFAP) following different treatments. (E, F) statistical analysis on Western blot results. Intrathecal infusion of TLR 3 ASO, but not TLR 3 MO, significantly blocked CP-induced TLR 3 up-regulation and astrocytic activation. *, # P < 0.05 compared to sham-MO or TNBS-MO group, respectively.