Table 1 Primers sequence for the rat genes characterized in this experiment
From: Spinal toll like receptor 3 is involved in chronic pancreatitis-induced mechanical allodynia of rat
Genes | Primers | Â | Accession number |
|---|---|---|---|
MCP-1 | Forward primer | 5'-CAGATCTCTCTTCCTCCACCACTAT-3' | M57441 |
| Â | Reverse primer | 5'-CAGGCAGCAACTGTGAACAAC-3' | Â |
IL-1β | Forward primer | 5'-TGCTGATGTACCAGTTGGGG-3' | NM031512 |
| Â | Reverse primer | 5'-CTCCATGAGCTTTGTACAAG-3' | Â |
IL-6 | Forward primer | 5'-GCCCTTCAGGAACAGCTATG-3' | NM012589 |
| Â | Reverse primer | 5'-CAGAATTGCCATTGCACAAC-3 | Â |
TNF-α | Forward primer | 5'-TGATCGGTCCCAACAAGG A-3' | AY427675 |
| Â | Reverse primer | 5'-TGCTTG GTG GTTTGCTACGA-3' | Â |
COX-2 | Forward primer | 5'-CAGTATCAGAACCGCATTGCC-3' | U03389 |
| Â | Reverse primer | 5'-GAGCAAGTCCGTGTTCAAGGA-3' | Â |
GAPDH | Forward primer | 5'-CCCCCAATGTATCCGTTGTG-3' | NM01008 |
| Â | Reverse primer | 5'-TAGCCCAGGATGCCCTTTAGT-3' | Â |