Expression profile of microRNAs in DRG and spinal dorsal horn after sciatic nerve axotomy. (A) MiR-1 (white bars) and -206 (black bars) showed a robust upregulation in the dorsal root ganglion whereas miR-16 (gray bars) relative expression also increased, but only on days 1 and 3 post-injury returning to its normal values on day 7 post-nerve lesion. (B) in the dorsal horn of the spinal cord, miR-1 was downregulated over the entire period of investigation and no change was observed for miR-16 and -206. Bars represent mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 for each miRNA expression in axotomized group compared to sham-operated control animals euthanized 24 h after surgery (time 0), n = 6 - 8 per group, Student's t-test.