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Figure 11 | Molecular Pain

Figure 11

From: Ret-dependent and Ret-independent mechanisms of Gfl-induced sensitization

Figure 11

ARTN-induced enhancement in the stimulated-release of iCGRP is mediated, in part, by Fyn-dependent pathways. A) This representative Western blot demonstrates that exposure of DRG to Fyn siRNA decreases Fyn levels, while Scramble siRNA does not change Fyn levels. B) Densitometric analysis of three separate Western blots like that in A probing for Fyn. C) This representative Western blot demonstrates that exposure of DRG to Fyn siRNA (100 nM) and scramble siRNA (100 nM) do not change c-Src levels. D) Densitometric analysis of three separate Western blots like that in A probing for c-Src. E and F) Peptide release elicited by a 10 minute exposure to Hepes buffer alone (open bar) or Hepes buffer containing 50 nM capsaicin (Cap; dark bars) is expressed as mean percent total peptide content of cells in each well ± SEM (n = 12-18 wells per condition). Asterisks (*) indicate statistically significant differences in band density and iCGRP release between treatment groups and the no GFL condition using an ANOVA with Dunnett's post-hoc test (p < 0.05). Ampersands (@) indicate statistically significant differences between the GFL treatment and the siRNA treated condition using a t-test (p < 0.05). In all cases, release stimulated by capsaicin was significantly higher than basal release.

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