Figure 2

ARTN-induced enhancement in the stimulated-release of iCGRP is mediated, in part, by NCAM-dependent pathways. A) This representative Western blot demonstrates that exposure of DRG to NCAM siRNA decreases NCAM levels, while scramble siRNA does not change NCAM levels. B) Densitometric analysis of three separate Western blots like that in A probing for NCAM. C) Peptide release elicited by a 10 minute exposure to Hepes buffer alone (open bar) or Hepes buffer containing 50 nM capsaicin (Cap; dark bars) is expressed as mean percent total peptide content of cells in each well ± SEM (n = 12-18 wells per condition). GFLs were included in the 10 minutes prior to and throughout capsaicin exposure. Asterisks (*) indicate statistically significant differences in band density and iCGRP release between treatment groups and the no GFL condition using an ANOVA with Dunnett's post-hoc test (p < 0.05). Ampersands (@) indicate statistically significant differences between the GFL treatment and the siRNA treated condition using a t-test (p < 0.05).