Figure 5

C fibre nociceptor mechanical responses after galanin and Gal2-11 treatment in a model of neuropathic pain. In PSNI (day three) nerve injured rats, 100 nM galanin and Gal2-11 s.c. enhanced mechanical activity in nociceptive afferents, whereas 10 uM galanin and Gal2-11 s.c. inhibited mechanical activity. [A] 100 nM galanin significantly reduced mechanical thresholds (one way ANOVA with post-hoc Bonferroni multiple comparison test, *p < 0.05, n = 5), and similarly [B] 100 nM Gal2-11 significantly reduced mechanical thresholds (one way ANOVA with post-hoc Bonferroni multiple comparison test, ** p < 0.01, n = 3). [C] 10 uM galanin significantly increased mechanical activation thresholds (one way ANOVA with post-hoc Bonferroni multiple comparison test, **p < 0.01, n = 4), and similarly [D] 10 uM Gal2-11 significantly increased mechanical activation thresholds (one way ANOVA with post-hoc Bonferroni multiple comparison test, * P < 0.05, n = 5).