Effect of PEA on λ-carrageenin-induced NGF expression and release in granulomatous tissue r 96 h after PEA administration. (A) Representative Western blot analysis and relative densitometric analysis of NGF. Tubulin expression is shown as a control. (B) ELISA assay of NGF release in granulomatous tissue. Data are representative of 3 separate experiments. Results are expressed as mean ± SEM of 3 experiments.; *p < 0.05, **p < 0.01, ***p < 0.001 vs. saline.; °p < 0.05, °°p < 0.01, °°°p < 0.001 vs. λ-carrageenin alone.