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Figure 2 | Molecular Pain

Figure 2

From: TRESK channel contribution to nociceptive sensory neurons excitability: modulation by nerve injury

Figure 2

In vitro and axotomy produces similar changes than in vivo axotomy. A. Acute dissociation of DRG sensory neurons produces similar hyperexcitability in previously injured or non-injured neurons. Left: Examples of spikes fired by a neuron axotomized in the animal and later dissociated and another cell only acutely dissociated. Right: Quantification of number of spikes fired, input resistance, resting membrane potential and action potential threshold. **p < 0.01 t-test Dissociated (Diss; n = 16) vs. Axotomized+dissociated (Axo+Diss; n = 15) cells. B. Effects of in vitro axotomy in total whole-cell currents. Currents elicited by a voltage ramp (-110 to +50 mV) in Diss. and Diss+Axo neurons (n = 7 for each group) in the presence of 2 μM TTX. Quantification did not show significant differences in currents at -110 or +50 mV. C. Examples of recordings in Diss. and Diss+Axo neurons using a protocol to minimize activation of voltage-gated transient K+ outward currents. Quantification of currents at the end of the pulse at -35 mV (arrow) and at the end of the ramp (-135 mV; arrow) did not show significant differences among groups.

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