Figure 3

Positive injury markers in dissociated and axotomized neurons. A. Upregulation of injury marker ATF3 in neurons axotomized in vivo (***p < 0.001; n = 4 animals). B. ATF3 shows similar levels of expression in Diss. and Diss+Axo neurons (n = 3 independent dissociated cultures). Notice that a similar fold change is obtained when normalizing with Diss. expression levels, implying that expression levels of Diss. neurons had increased to levels in axotomized neurons. Cacna2d1 and TRESK expression also followed the same pattern (compare data in Fig 3B and Fig 1B) although a significant decrease in TRESK is still seen in Diss+Axo neurons compared to Diss. neurons only (*p < 0.05; n = 4 independent experiments).