Figure 1
From: Nav1.7 is the predominant sodium channel in rodent olfactory sensory neurons
Restriction analysis of multiplex PCR amplicons of sodium channels from adult rat and mouse olfactory epithelium. (A) Lane M shows 100-bp ladder marker, and lane 1 contains amplicon from rat olfactory epithelium. Bands "a", "b" and "c" (best seen in Lane 8) are consistent with the presence of a potential mixture of sodium channels. Band "a": Nav1.1 (558 bp), Nav1.2 (561 bp) and Nav1.3 (561 bp), band "b": Nav1.5 (519 bp); band "c": Nav1.6 (507 bp), Nav1.7 (501 bp), Nav1.8 (480 bp), Nav1.9 (468 bp) and Nax (501 bp). Lanes 2-11 show results of cutting this DNA with EcoRV (Nav1.1), EcoNI (Nav1.2), AvaI (Nav1.3), NaeI (Nav1.4/Nax), AccI (Nav1.5/1.9), SphI (Nav1.6), BamHI (Nav1.7/1.8), AflII (Nav1.8), EcoRI (Nav1.9), and XbaI (Nax). (B) Lane M shows 100-bp ladder marker, and lane 1 contains amplicons from mouse olfactory epithelium. Bands "a" and "b" are consistent with the presence of a potential mixture of sodium channels. Band "a": Nav1.1 (558 bp), Nav1.2 (561 bp) and Nav1.3 (561 bp); band "b": Nav1.6 (510 bp), Nav1.7 (501 bp), Nav1.8 (480 bp), Nav1.9 (471 bp) and Nax (501 bp). Lanes 2-11 show the results of cutting this DNA with EcoRV (Nav1.1), EcoNI (Nav1.2), DraI (Nav1.3), PvuI (Nav1.4), AgeI (Nav1.5), SphI (Nav1.6), ScaI (Nav1.7), AhdI (Nav1.8), EcoRI (, Nav1.9) and AlwNI (Nax).