Figure 7

Repeated administrations of 2ccPA (i.v. or i.t.) induces analgesia against established thermal hyperalgesia in mice with neuropathic pain. A, Neuropathic pain in mice was induced by partial sciatic nerve injury. Vehicle or 2ccPA (i.v. or i.t.) was injected daily for 7 days, starting 7 days after injury. B, Vehicle or 2ccPA (100 nmol/5 μL 1-3 days and 10 nmol/5 μL 4-7 days, respectively, i.t.) was daily injected for 7 days, starting 7 days after injury. The withdrawal latency was measured at 30, 60, 90, and 120 min after the injection of 2ccPA (i.t.) on the first (a), fourth (b), or seventh day (c). The basal latency was measured before injection of 2ccPA (i.t.) (d). (C) Vehicle or 2ccPA (10 mg/kg, i.v.) was daily injected for 7 days, starting 7 days after injury. The withdrawal latency was measured at 30, 60, 90, and 120 min after the injection of 2ccPA (i.v.) on the first (a), fourth (b), or seventh day (c). The basal latency was measured before injection of 2ccPA (i.t.) (d). All data represent the mean ± S.E. from 3-6 individual mice per group. *P < 0.05 indicates significant difference from the vehicle response by Tukey's multiple comparison test.