Figure 9

Effects of i.v. 2ccPA on thermal paw-withdrawal and mechanical allodynia of nerve-ligated rats. A, The rat sciatic nerve was ligated 7 days prior to i.v. injection of 2ccPA, applied once daily for 7 consecutive days. B, A hind paw was irradiated with infrared light to evoke thermal withdrawal, and the time variation of the withdrawal latency was measured prior to and at 10 min, 2 h, or 4 h after the application of 2ccPA on the seventh day. C, Dose response; 2-h injection of 2ccPA at 3 or 10 mg/kg applied once daily for 7 consecutive days. D, Time course; 2-h injection of 2ccPA (10 mg/kg) applied once daily for 4 or 7 consecutive days. Gabapentin (90 mg/kg i.v.) was applied as a control. *P < 0.05 and #P < 0.05 indicates significant difference from the vehicle response by Dunnett's test and by Student's t-test or Welch's test, respectively. E, The responses of rats to tactile stimulation was tested with 5 von Frey filaments, and threshold filament sizes to evoke paw withdrawal were measured prior to and at 10 min, 2 h, or 4 h after application of 2ccPA on the seventh day. F, Dose response; 4-h injection of 2ccPA at 1, 3 or 10 mg/kg applied once daily for 7 consecutive days. G, Time course; 4-h injection of 2ccPA (10 mg/kg) applied once daily for 4 or 7 consecutive days. Gabapentin (90 mg/kg i.v.) was applied as a control. *P < 0.05 and #P < 0.05 indicates significant difference from the vehicle response by Steel's test and by Wilcoxon test, respectively.