Figure 3

Calmodulin inhibition increases AKAP150- mediated sensitization of TRPV1 activity. (A-B) Representative traces of CAP-stimulated (50 nM, 30 sec) intracellular Ca²⁺ accumulation from CHO cells expressing TRPV1 and AKAP150. Cells pretreated with vehicle (H₂0, veh) or W-7 (100 μM) or CAIP (50 μM) for 30 min followed by 8-Br-cAMP (10 μm, 30 sec) administration. (C) Data represented in A are quantified as ΔF340/F380, mean ± SEM shown, n shown for each treatment paradigm. The white § indicates sample groups pre-treated with calcineurin auto-inhibitory peptide (CAIP). Significance determined by one-way ANOVA with Bonferroni correction, *p < 0.01, ***p < 0.001.